The optimization of in vitro multi organ cell-based assays to evaluate the impact of complex foods on human health is a hot topic in nutrition research.

The testing of health effects of a high number of foods, combined in complex meals with a high number of molecules of interest, relies on the availability of experiments in high-throughput, physiomimetic and highly controlled conditions.

Our group has been successfully optimizing the integration of gastrointestinal digestion simulation (bioaccessible fractions), with in vitro gastric and intestinal transport (bioavailable fraction), and the bioactivity (both deleterious and beneficial) in several target organs simulated in culture of human cell lines, as showed in listed publications.

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Mycotoxin Interactions along the Gastrointestinal Tract: In Vitro Semi-Dynamic Digestion and Static Colonic Fermentation of a Contaminated Meal.

Toxins 2022, 14(1):28. https://doi.org/10.3390/toxins14010028

Effect of skimmed milk on intestinal tract: prevention of increased reactive oxygen species and nitric oxide formation.

International Dairy Journal 2021, 118, 105046. https://doi.org/10.1016/j.idairyj.2021.105046

Fat Oxidation of Fatty Fish vs. Meat Meal Diets Under in vitro Standardized Semi-Dynamic Gastric Digestion.

Frontriers in Nutrition 2021, 9:901006.https://doi.org/10.3389/fnut.2022.901006