FOODinteract Research Team (I3A-LAQV)

An indirect in-house ELISA targeting caseins (Bos d 8) is proposed as a highly specific and sensitive method, allowing detecting minute amounts of milk protein (10 mg/kg) in processed meat products (cooked hams and sausages). A second indirect ELISA is advanced for β-lactoglobulin (Bos d 5) detection presenting different sensitivities (100-1000 mg/kg), depending on the matrix and the state of food processing.

Bos d 8 ELISA has great potential to detect milk proteins in highly processed foods due to caseins stability, whereas Bos d 5 ELISA is useful to analyse food formulations enriched with whey proteins. Both proposed ELISA use commercial antibodies, providing cost-effective alternatives to the available ELISA kits.

HIGHLIGHTS (Elisa)